Part:BBa_K5415003:Design

Nonuple HRE biosensor with RFP for severe hypoxia detection.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1
  Illegal XhoI site found at 21
  Illegal XhoI site found at 35
  Illegal XhoI site found at 55
  Illegal XhoI site found at 69
  Illegal XhoI site found at 89
  Illegal XhoI site found at 103
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Three sets of triple HRE containging oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeCherry:

ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA

GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT

ATGTACGGTAGCAAGGCATACGTCAAGCACCCAGCTGACATTCCAGATTATTTGAAATTATCTTTTCCTGAAGGGTTTAAATGGGAAAGAGTTATGAATTTTGAG

GATGGTGGTGTTGTCACTGTCACACAAGACTCCAGTTTACAAGATGGTGAGTTCATCTACAAGGTTAAATTGAGAGGTACTAACTTTCCATCAGACGGTCCTGTT

ATGCAAAAGAAAACTATGGGTTGGGAAGCTAGCTCCGAAAGAATGTATCCTGAAGATGGTGCTTTGAAGGGTGAAATTAAGCAAAGATTGAAATTGAAAGATGGT

GGTCATTATGATGCAGAAGTTAAGACAACGTATAAAGCGAAAAAACCAGTTCAGCTTCCAGGTGCTTATAATGTTAATATTAAGTTGGACATTACCTCTCATAAT

GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata

ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt

tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat